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目的 :了解砷摄取与组织器官巯基含量关系。方法 :用慢性动物实验 ,检测饲以含砷 80mg/kg饲料小白鼠器官组织活性巯基含量 ,分析砷摄入与巯基含量关系。结果 :第一 ,实验组血清巯基含量为 2 9.48± 4.60 mg/L,明显高于对照组的 2 2 .41± 2 .79mg/L( P<0 .0 1 )。实验组毛砷为对照组的 6倍。血清巯基含量随着体毛砷含量上升而上升 ( P<0 .0 5 )。第二 ,实验组肌肉砷含量为对照组的 5 .4倍 ,但实验组的巯基含量低于对照组 ( P<0 .0 5 ) ,在肌肉中砷含量与巯基含量呈反比关系 ( P<0 .0 5 )。第三 ,实验组所观察的器官 (肝、心、肾、肺、脾和脑 )的巯基水平都低于相应对照组 ,在肺、心和脑呈现明显负相关 ( P<0 .0 5 )。结论 :砷摄入虽然使血清巯基水平上升 ,但抑制器官和组织的巯基含量 ,组织器官巯基含量下降可能为砷对组织巯基长期作用的结果
Objective: To understand the relationship between arsenic uptake and the content of sulfhydryl groups in tissues and organs. Methods: Chronic animal experiments were used to detect the content of active thiols in the organs of mice fed with arsenic 80mg / kg, and the relationship between the arsenic uptake and the content of sulfhydryl groups was analyzed. Results: First, the content of sulfhydryl in the experimental group was 2 9.48 ± 4.60 mg / L, which was significantly higher than that of the control group (22.41 ± 2.79 mg / L, P <0.01). Experimental group hair arsenic 6 times the control group. Serum thiol content increased with the increase of body hair arsenic (P <0.05). Second, the muscle arsenic content in the experimental group was 5.4 times that of the control group, but the content of sulfhydryl group in the experimental group was lower than that in the control group (P <0.05), and the content of arsenic in the muscle was inversely proportional to the sulfhydryl content (P < 0 .0 5). Third, the levels of sulfhydryl in organs (liver, heart, kidney, lung, spleen and brain) observed in the experimental group were lower than those in the corresponding control group, and there was a significant negative correlation between lung, heart and brain (P <0.05) . CONCLUSIONS: Although arsenic uptake increases the level of serum thiol, inhibition of the sulfhydryl content of organs and tissues and the decrease of the sulfhydryl content of tissues and organs may be the result of a long-term effect of arsenic on tissue sulfhydryl