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为克服有性杂交障碍 ,利用原生质体电融合技术开展杨树体细胞融合研究。本研究选择杨树不同种或无性系分离原生质体 ,并应用IOA和X 射线分别对融合双亲处理 ,使其失去分裂能力 ,筛选杂种细胞 ,并探索杨树原生质体融合的最适电场参数与培养条件 ,建立杨树体细胞电融合体系。首次得到了美洲黑杨 +胡杨、青杨 +胡杨、美洲黑杨 +青杨等体细胞杂种愈伤组织。其中美洲黑杨 +胡杨、青杨 +胡杨体细胞杂种愈伤组织可在附加 0 .4%氯化钠的培养基上生长 ,表现出融合亲本之一胡杨的耐盐特性
In order to overcome the obstacles of sexual hybridization, poplar somatic cell fusion was carried out by protoplast electrofusion technology. In this study, protoplasts from different clones or clones of poplar were isolated and the hybrid parents were treated with IOA and X-ray respectively to lose their ability to split and select the hybrid cells. The optimum electric field parameters and culture of poplar protoplast fusion Conditions, the establishment of poplar somatic electrical fusion system. Populus euphratica + Populus euphratica, Populus euphratica + Populus euphratica and Populus tomentosa were the first to get somatic hybrid callus. Populus euphratica + Populus euphratica, Populus euphratica + Populus euphratica hybrid callus can grow on the medium supplemented with 0.4% sodium chloride, showing salt tolerance of Populus euphratica