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目的 L -精氨酸·L门冬氨酸盐具有抑制血小板聚集和血栓形成的作用 ,本文观察它在体外对GPIIb IIIa单抗FITC PAC 1与洗涤家兔血小板结合的影响和体内给药对大鼠血小板活性物质的影响 ,以探讨其作用机制。方法 用流式细胞仪测定抗体与活化血小板的结合 ;用比色法测定血清NO浓度 ;用放免法测定cAMP ,TXA2 和PGI2 水平。结果 L -精氨酸·L门冬氨酸盐 3 0mg·kg- 1灌胃给药 ,可明显增加大鼠血浆NO浓度和大鼠主动脉段体外培养上清液中 6 keto PGF1α水平 ,但对血浆中TXB2 和 6 keto PGF1α水平和血小板内cAMP的含量无明显影响 ,而乙酰水杨酸则明显降低血清TXB2 和 6 keto PGF1α水平。L -精氨酸·L门冬氨酸盐 10 0 μmol·L- 1在体外可明显减少GPIIb IIIa单抗FITC PAC 1与血小板的结合。结论 L -精氨酸·L-门冬氨酸盐抑制血小板聚集和抗血栓形成的功效可能通过增加血管内皮细胞释放NO和PGI2 ,继而阻止血小板活化来介导 ,而与花生四烯酸及cAMP代谢途径无密切关系
Objective L-arginine · L-aspartate has the effect of inhibiting platelet aggregation and thrombosis. In this paper, we observed its effect on the binding of GPIIb IIIa monoclonal antibody FITC PAC 1 to the washed rabbit platelets in vitro and the effect of its administration in vivo Rat platelet active substances to explore its mechanism of action. Methods The binding of antibodies to activated platelets was measured by flow cytometry. The concentration of serum NO was measured by colorimetric method. The levels of cAMP, TXA2 and PGI2 were determined by radioimmunoassay. Results The intragastric administration of L - arginine · L - aspartate at 30 mg · kg -1 could significantly increase the concentration of NO in rat plasma and the level of 6 keto PGF1α in the culture supernatant of rat aorta, TXB2 and 6 keto PGF1α levels in plasma and platelet cAMP content had no significant effect, while acetylsalicylic acid was significantly lower serum TXB2 and 6 keto PGF1α levels. L-arginine · L-aspartate 10 0 μmol·L -1 significantly reduced the binding of GPIIb IIIa monoclonal antibody FITC PAC 1 to platelets in vitro. Conclusion The inhibitory effect of L - arginine and L - aspartate on platelet aggregation and antithrombosis may be mediated by increasing the release of NO and PGI2 by vascular endothelial cells and then the inhibition of platelet activation. However, the effects of alanine and cAMP Metabolic pathway is not closely related