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目的 :探讨声损伤后鸡基底乳头 (BP)感觉上皮细胞的增殖活动特点和再生毛细胞的前体细胞来源。方法 :采用 1.5kHz、12 0dBSPL的纯音连续刺激 4 8h ,造成鸡内耳声损伤。于声刺激开始后 4 0h及 4 8h ,活体引入细胞增殖标记物———溴脱氧尿苷 ,6h后处死动物并制备石蜡切片 ,然后进行免疫细胞化学染色 ,观察BP中的细胞增殖活动。结果 :①BP中细胞增殖活动出现的位置与声损伤部位一致。② 4 8h前后 ,BP中的细胞增殖活动最活跃。③标记支持细胞和标记毛细胞均可见 ,支持细胞可能是再生毛细胞的前体细胞。结论 :声损伤后鸡BP感觉上皮细胞存在活跃的增殖活动 ,并通过毛细胞再生达到BP形态结构的修复 ,支持细胞可能是再生毛细胞的前体细胞
Objective: To investigate the proliferative characteristics of sensory epithelial cells in basilar papillae (BP) and the source of precursor cells of regenerative hair cells after acoustic injury. Methods: The pure tone of 1.5kHz and 12 0dB SPL was stimulated continuously for 48h, resulting in acoustic damage to the inner ear of chicken. After 4h and 48h after the start of sonic stimulation, the cell proliferation marker bromodeoxyuridine was introduced into the living body. After 6h, the animals were sacrificed and paraffin sections were prepared. Immunocytochemical staining was then performed to observe the cell proliferative activity in BP. Results: ① The location of cell proliferative activity in BP was consistent with the location of acoustic injury. 48h before and after, the cell proliferation in BP most active. ③ labeled support cells and labeled hair cells are visible, supporting cells may be regenerating hair cells precursor cells. CONCLUSION: There is active proliferative activity in sensory epithelial cells of chicken BP after acoustic injury, and the repair of BP morphological structure can be achieved by hair cell regeneration. The supporting cells may be the precursors of regenerating hair cells