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目的对肝素抗凝血浆用于急诊生化检验的临床可行性进行临床分析。方法对100例患者的血清及肝素抗凝血浆的10项急诊生化项目进行测定,并采用日立7600对所采集样本进行全自动生化分析测定。结果在10项急诊生化项目进行测定中样本血清与血浆中氯离子(Cl~-)、钙离子(Ca~(2+))、镁离子(Mg~(2+))、尿素氮(BUN)、肌酐(Cr)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的检测结果比较差异均无统计学意义(P>0.05)。血清与血浆中钾离子(K~+)、钠离子(Na~+)、葡萄糖(Glu)的检测结果比较[(4.09±0.29)mmol/L VS(3.76±0.35)mmol/L、(135.3±2.9)mmol/L VS(138.2±3.5)mmol/L、(4.73±1.41)mmol/L VS(5.39±1.41)mmol/L]差异均具有统计学意义(P<0.05)。对血清中的K~+浓度(Y)与血浆中的K~+浓度(X)做回归方程为Y=1.005X+0.19,r=0.998。结论肝素抗凝血浆可以用于检验10项急诊生化项目,但应注意血清中的K~+浓度与血浆中的K+浓度存在一定的恒定的浓度差约为0.227 mmol/L,在临床测定时应注意。
Objective To analyze the clinical feasibility of heparin anticoagulated plasma for emergency biochemical tests. Methods The 10 emergency biochemical items of serum and heparin anticoagulated plasma in 100 patients were measured. The samples collected by Hitachi 7600 were determined by automatic biochemical analysis. Results The levels of Cl ~ -, Ca ~ (2+), Mg ~ (2+), BUN in serum and plasma were determined in 10 emergency biochemical projects. , Creatinine (Cr), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the control group had no significant difference (P> 0.05). (4.09 ± 0.29) mmol / L vs (3.76 ± 0.35) mmol / L, (135.3 ± 0.45) mmol / L in plasma and plasma, 2.9 mmol / L VS 138.2 ± 3.5 mmol / L and 4.73 ± 1.41 mmol / L VS 5.39 ± 1.41 mmol / L, respectively) were statistically significant (P <0.05). The regression equation of K + concentration (Y) in serum and K + concentration in plasma (X) was Y = 1.005X + 0.19, r = 0.998. Conclusion Heparin anticoagulant plasma can be used to test 10 emergency biochemical projects, but it should be noted that there is a certain constant concentration difference between K + concentration in serum and K + concentration in plasma about 0.227 mmol / L, note.