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Objective To study the characteristic 2-(2-phenylethyl)chromone components of endophytic fungal strain of Aquilaria sinensis by solid culture. Methods The compounds were isolated by various chromatographic methods such as silica gel, reverse-phase silica gel, Sephadex-LH20 column chromatography as well as crystallization. Results Seven 2-(2-phenylethyl)chromone analogues were isolated from the solid culture of Botryosphaeria rhodina A13. Their structures were established by spectral data as well as physicochemical properties, and identified as 6-hydroxy-7-methoxy-2-(2-phenylethyl)chromone(1), 6,7-dimethoxy-2-(2-phenylethyl)chromone(2),(5S,6R,7S,8R)-2-(2-phenylethyl)-5,6,7,8-tetrahydrchromone(3), 6-hydroxy-2-(2-phenylethyl)chromone(4), 4′-hydroxy-2-(2-phenylethyl)chromone(5), 6-methoxy-2-phenethyl-4H-chromen-4-one(6), and 6-methoxy-2-(4′-methoxyphenethyl)-4H-chromen-4-one(7). Conclusion All of the compounds are isolated for the first time from the genus Botryosphaeria. This research opens up a new vista to produce the characteristic components of agarwood by endophytic fungi.
Objective To study the characteristic 2- (2-phenylethyl) chromone components of endophytic fungal strain of Aquilaria sinensis by solid culture. Methods The compounds were isolated by various chromatographic methods such as silica gel, reverse-phase silica gel, Sephadex-LH20 column chromatography Results Seven 2- (2-phenylethyl) chromone analogues were isolated from the solid culture of Botryosphaeria rhodina A13. Their structures were established by spectral data as well as physicochemical properties, and identified as 6-hydroxy-7-methoxy (2-phenylethyl) chromone (2), (5S, 6R, 7S, 8R) -2- , 6,7,8-tetrahydrchromone (3), 6-hydroxy-2- (2-phenylethyl) chromone (4) -phenethyl-4H-chromen-4-one (6), and 6-methoxy-2- (4’-methoxyphenethyl) -4H- chromen-4-one (7). Conclusion All of the compounds are isolated for the first time from the genus Botryosphaeria. This research opens up a new vista to produce the characteristic components of agarwood by endophytic fungi.