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目的:建立舒血灵胶囊中三七皂苷R1、人参皂苷Rg1及人参皂苷Rb1的含量测定方法。方法:采用Hypersil ODS-2C18(250 mm×4.6 mm,5μm)色谱柱;以乙腈(A)-水(B)为流动相进行梯度洗脱,流动相梯度为:0~8 min,20%A→20%A,8~40 min,20%A→30%A,40~60 min,30%A→45%A;流速:1.0 ml·min-1;柱温:25℃;检测波长:203 nm;进样量:20μl。结果:三七皂苷R1在浓度0.05~0.50 mg·ml-1范围内,人参皂苷Rg1和Rb1在浓度0.20~2.00 mg·ml-1范围内,与峰面积呈较好的线性关系(r=0.999 9);三种成分的平均加样回收率分别为98.79%、98.42%、98.89%,RSD分别为0.85%、0.97%、0.74%(n=6);日内精密度分别为0.49%、0.20%和0.39%;日间精密度分别为0.75%、0.56%和0.51%;稳定性、重复性试验的RSD<1%。结论:本试验建立的测定方法简便、准确性高、重复性好,可用于该制剂的含量测定。
Objective: To establish a method for the determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Shu Xie Ling capsule. Methods: The column was eluted with Hypersil ODS-2C18 (250 mm × 4.6 mm, 5 μm). The mobile phases were acetonitrile (A) -water (B) → 20% A, 8-40 min, 20% A → 30% A, 40-60 min, 30% A → 45% A; flow rate: 1.0 ml · min -1; nm; injection volume: 20μl. Results: The concentrations of ginsenoside R1 in the range of 0.05-0.50 mg · ml-1 and the concentrations of ginsenosides Rg1 and Rb1 in the range of 0.20-2.00 mg · ml-1 showed a good linear relationship with the peak area (r = 0.999 9). The average recoveries of the three components were 98.79%, 98.42% and 98.89%, respectively. The RSDs were 0.85%, 0.97% and 0.74%, respectively (n = 6) And 0.39%, respectively. The daytime precision was 0.75%, 0.56% and 0.51%, respectively. The RSDs of stability and repeatability were less than 1%. Conclusion: The test method established in this study is simple, accurate, reproducible, and can be used for the determination of the content of the preparation.