论文部分内容阅读
优化玫瑰红鹅膏液体发酵培养条件,并研究其对植物病原菌活性的抑制作用。采用中心组合旋转设计法确定玫瑰红鹅膏液体深层培养菌丝体的最佳条件,采用HPLC和ESI-MS法对其发酵产物中的肽类毒素进行检测。采用生长速率法研究玫瑰红鹅膏子实体粗毒液和发酵产物对香瓜枯萎病菌的抑制作用。当温度25℃、pH 6、转速156r·min-1时,菌丝体重量达到最高,为1.054 g。从发酵产物中分离鉴定出2种化合物:α-AMA和β-AMA。玫瑰红鹅膏子实体粗毒液和发酵产物对香瓜枯萎病菌有很好的抑制作用,玫瑰红鹅膏子实体粗毒液浓度为200 g·mL-1时,抑制率达最高为42.47%。玫瑰红鹅膏发酵产物中检测出2种肽类毒素:α-AMA和β-AMA;玫瑰红鹅膏子实体能有效的抑制香瓜枯萎病菌的活性,具有开发利用价值。
The liquid fermentation culture conditions of rose red amanita were optimized and their inhibitory effects on the activity of plant pathogenic bacteria were studied. The optimum conditions for deep culture of Mycelia roseum were determined by center-combination rotational design method. The peptide toxins in the fermentation products were detected by HPLC and ESI-MS. The growth rate method was used to study the inhibitory effect of crude venom and fermentation products of rose red goose angelica on Fusarium oxysporum f. Sp. When the temperature was 25 ℃, pH 6, rotation speed 156r · min-1, mycelium weight reached the highest, 1.054 g. Two compounds were identified from fermentation products: α-AMA and β-AMA. The results showed that the crude venom and the fermentation products of R. roseum had a good inhibitory effect on Fusarium oxysporum. The highest inhibitory rate was 42.47% at the crude venom concentration of 200 g · mL-1. Two kinds of peptide toxins were detected in the fermentation products of rose red amanita: α-AMA and β-AMA. The rose red goose ghost fruiting bodies could effectively inhibit the activity of Fusarium oxysporum f. Sp.