论文部分内容阅读
作者在我国新发现的山西恙虫病疫区病人血液中分离出3株恙虫病立克次体,SXh951,Sxh952,Sxh953.为明确其分类地位.对其进行了血清型,基因型和56－kD蛋白基因的部分序列分析.用免疫荧光抗体染色法将病人血清抗体与Karp,Gilliam.Kato株抗原反应.结果显示山西株与Gilliam型一致;用来自56－kD蛋白基因的引物扩增后,分别用限制性核酸内切酶Hinfl,HaeⅢ.Hhal消化DNA片段的多态性(PCR/RFLP)显示山西3株基因型一致,但均与Karp,Kato,Gilliam株的电泳图谱不同,显示为一独有的PCR/RFLP基因型;对Sxh951株56-kD蛋白基因片段扩增产物(1.2kb)进行DNA序列分析后.根据所推导氨基酸序列,比较Sxh951株与三个标准株氨基酸序列的同源性,发现Sxh951株与Karp、Kato和Gilliam株均有明显差异.对此,本实验室正在进一步研究,以便进一步确定其分类地位.“,”Three R. tsutsugamushi strains were newly isolated from patients in Shanxi province. In order to identify the phylogeny of the three R. tsutsugamushi strains,analysis was taken in its serology,genotype and Partial nucleotide sequence encoding 56-kD protein. The three isolates were identical with Gillian strain in serology with IF method which Karp,Gilliam and Kato strain antigen reacted with patients sera. At the same time they were identical in genotype with PCR/RFLP method which PCR products were digested with endonuclease Hae M, HhaI, HinfI respectively. but the PCR/RFLP pattern of these isolates were different from Karp, Kato nad Gilliam strains. The partial nucleotide sequence encoding 56-kD protein gene was also determined. The size of the sequence was about 1200 base pair,and the amino acid sequence encoded by the gene fragment was deduced. The homology between Sxh951 and Karp,Gilliam,Kato was diverse. Further studies will be carried out in our lab in order to identify its phylogeny.