论文部分内容阅读
目的:研究健骨固本胶囊(GB)含药血清对成骨细胞(OB)的作用及可能作用机制。方法:用胰蛋白酶-胶原酶消化法分离培养新生SD大鼠颅盖骨OB,以GB含药血清的培养液进行培养,分析含药血清对OB增殖、碱性磷酸酶(ALP)活性、钙化能力及OB的雌激素受体α(ER-α)表达的影响。结果:GB含药血清能刺激OB增殖、提高ALP的活性、增加OB的钙化能力,上调OB的ER-α表达,与空白对照组比较差异显著。结论:GB含药血清能刺激OB的增值、增加钙化能力,其机制可能与上调OB的ER-α表达有关。
Objective: To study the effect of GBJ-containing serum on osteoblasts (OB) and its possible mechanism. Methods: The calvaria OB of newborn SD rats was isolated and cultured by trypsin-collagenase digestion method. The serum OB was cultured in the culture medium containing GB serum. The effects of serum containing on OB proliferation, ALP activity, calcification Abilities and the expression of estrogen receptor alpha (ER-α) in OB. Results: The serum containing GB could stimulate the proliferation of OB, increase the activity of ALP, increase the calcification ability of OB, and up-regulate the expression of ER-α in OB, which is significantly different from the blank control group. CONCLUSION: GB-containing serum can stimulate the added value of OB and increase the calcification ability, which may be related to the up-regulation of ER-α expression in OB.