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目的:探究白花蛇舌草对宫颈癌的抑制作用及可能的分子生物学机制。方法:建立裸鼠人宫颈癌细胞移植模型及采用胃内灌药。当肿瘤生长至直径10mm时,将荷瘤鼠随机分组,比较给药组与对照组在抑瘤率、生存时间、HeLa细胞Ki-67抗原蛋白的表达率以及肿瘤组织凋亡上的差异。结果:白花蛇舌草对HeLa细胞移植瘤生长有明显抑制作用,并可诱导HeLa细胞凋亡,Ki-67蛋白的表达下降(P<0.05),并明显延长荷瘤小鼠的平均生存时间(P<0.05)。结论:白花蛇舌草通过直接抑瘤增殖和诱导、促进肿瘤细胞凋亡,实现了其清热解毒、软坚散结的作用。
Objective: To investigate the inhibitory effect of Hedyotis diffusa on cervical cancer and its possible molecular mechanism. Methods: To establish a human cervical cancer cell transplantation model in nude mice and intragastric infusion. When the tumor grew to a diameter of 10mm, the tumor-bearing mice were randomly divided into groups and compared the tumor inhibition rate, survival time, the expression rate of Ki-67 antigen protein in HeLa cells and the apoptosis of tumor tissue between the treated group and the control group. Results: Hedyotis diffusa could significantly inhibit the growth of HeLa cell xenografts and induce the apoptosis of HeLa cells. The expression of Ki-67 protein decreased (P <0.05) and prolonged the mean survival time of tumor-bearing mice P <0.05). Conclusions: Hedyotis diffusa will promote the apoptosis of tumor cells through direct inhibition of tumor proliferation and induction, and achieve the function of detoxification and endometriosis.