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利用研制开发的靶向性非病毒载体GE7基因导入系统转导外源基因pCEP-p21WAF-1, 检验其介导p21WAF-1基因进入肝癌细胞后, 在体内外抑制肝癌细胞的生长的效率. 体外实验证明, 导入外源基因后, SMMC-7721和BEL-7402细胞的生长受到抑制, 第8天抑制率分别达到56%和66.7%. 而体内实验结果是, 荷SMMC-7721裸鼠皮下瘤周注射该基因导入系统, 转导pCEP-p21WAF-1 3周后, 实验组肿瘤的重量(平均为(0.083 ( 0.043)g)与对照组(平均为(0.281 ( 0.173) g)相比明显减小, 且在统计学上有显著差别(P < 0.05). 以上结果表明, GE7基因导入系统可以介导外源基因pCEP-p21WAF-1进入细胞内, 并在体内外抑制肝癌细胞的生长.
The pCEP-p21WAF-1 gene was transducted by the introduced non-viral vector GE7 gene introduction system to test the efficiency of p21WAF-1 gene in inhibiting the growth of hepatoma cells in vitro and in vivo after it mediated the p21WAF-1 gene into hepatoma cells. The results showed that the growth of SMMC-7721 and BEL-7402 cells was inhibited after the introduction of exogenous gene, and the inhibition rates reached 56% and 66.7% respectively on the 8th day.The results of in vivo experiments showed that the SMMC-7721 subcutaneous tumor After 3 weeks of transduction of pCEP-p21WAF-1 gene into the system, the weight of tumor in the experimental group (mean (0.083 (0.043) g) was significantly reduced compared with the control group (average 0.281 (P <0.05) .The above results indicated that the introduction of GE7 gene into the system can induce the exogenous gene pCEP-p21WAF-1 into the cell, and in vitro and in vivo inhibit the growth of liver cancer cells.