由于免疫组化制片环节众多、周期长,且要经过高温、高压或酶消化、反复洗涤等环节,易出现脱片现象,特别是细胞涂片或组织极其微小的标本数量有限,组织脱失后难以复制,对病理诊断造成极大的影响。我们通过大量实践,改良了方法、优化了步骤,脱片现象明显改善,现总结如下。 Due to the large number of immunohistochemical production processes, long cycle, and to go through high temperature, high pressure or enzyme digestion, repeated washing and other links, prone to delamination phenomenon, especially cell smears or tissue is extremely small specimens a limited number of tissue loss After difficult to copy, the pathological diagnosis caused a tremendous impact. Through a great deal of practice, we have improved our methods, optimized the procedures and significantly improved the phenomenon of debinding, which is summarized as follows.