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目的建立反相高效液相色谱法测定人血浆中痂囊腔菌素A含量。方法血浆样品经处理后,采用RP-HPLC测定。色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),流动相为乙腈∶水(70∶30,V/V),流速1 mL.min-1,柱温27℃,检测波长470 nm。结果痂囊腔菌素A在0.300～10.000 mg.L-1范围内线性关系良好(r=0.999 3),定量限为0.300 mg.L-1。低、中、高浓度(0.500,3.000,8.000 mg.L-1)的日内及日间RSD≤7.37%,平均提取回收率分别为(93.97±6.32)%,(91.71±5.79)%和(88.95±3.26)%。结论本法简便、快捷、灵敏、准确,可满足临床试验中痂囊腔菌素A药动学研究的需要。
Objective To establish an RP-HPLC method for the determination of cystatin A in human plasma. Methods Plasma samples were processed and determined by RP-HPLC. The chromatographic column was Kromasil C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile: water (70:30, V / V) and the flow rate was 1 mL.min-1. Results Cystatin A had a good linearity (r = 0.999 3) in the range of 0.300-10.000 mg.L-1 with a limit of quantification of 0.300 mg.L-1. The intra-and inter-day RSDs of low, medium and high concentrations (0.500, 3.000 and 8.000 mg.L-1) were less than or equal to 7.37%, and the average recovery rates were (93.97 ± 6.32)%, (91.71 ± 5.79)% and ± 3.26)%. Conclusion This method is simple, rapid, sensitive and accurate, which can meet the needs of pharmacokinetics of cystatin A in clinical trials.