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应用空斑形成细胞（PFC）方法观察体外免疫脾细胞在应激血清作用下，分泌抗体的能力并与正常血清进行比较，结果表明免疫脾细胞在应激血清作用下分泌抗体的能力与对照组间差异无显著意义；但是应激血清可抑制脂多糖（LPS）刺激的淋巴细胞转化反应，且有量效关系。应用ConA活化的脾细胞制成IL－2反应细胞，在加入应激血清及正常对照血清作用下，观察IL－2反应细胞对重组基因IL－2（rIL－2）的反应性变化发现，当应激血清与rIL－2同时加入培养基时，IL－2反应细胞的增殖效应与对照组间差异无显著性意义；但是预先将应激血清与ConA活化的脾细胞混合培养2h后，再加入rIL－2，在应激血清作用下IL－2反应细胞对rIL－2刺激的反应性则较对照组显著降低；不同稀释度的应激血清的抑制作用也相应不同。说明应激血清可抑制ConA活化的脾细胞对IL－2刺激的反应性。
The use of plaque-forming cells (PFC) method to observe the ability of immune spleen cells in vitro to secrete antibodies under the action of stress serum and compared with normal serum. The results showed that the ability of immune spleen cells to secrete antibodies under the action of stress serum was significantly lower than that of the control group There was no significant difference between the two groups. However, the stress serum could inhibit the LPS-stimulated lymphocyte transformation reaction with dose-effect relationship. IL-2-responsive cells were prepared from ConA-activated spleen cells. The response of IL-2-responsive cells to recombinant IL-2 (rIL-2) was observed under the action of added stress serum and normal control serum. When the serum and rIL-2 were added to the medium at the same time, the proliferative effect of IL-2-reactive cells was not significantly different from that of the control group. However, when the serum was incubated with ConA-activated spleen cells for 2h, rIL-2, the responsiveness of IL-2-responsive cells to rIL-2 stimulated by stress serum was significantly lower than that of the control group; the inhibitory effects of different dilutions of stress serum were also different. It indicates that the stress serum can inhibit the reactivity of ConA-activated splenocytes to IL-2 stimulation.