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目的研究连翘抗病毒有效部位(LC-4)的提取分离及其体外抗呼吸道合胞病毒(RSV)的作用。方法将连翘水提液经水提醇沉法、大孔吸附树脂柱层析法,洗脱剂洗脱分离得到连翘抗病毒有效部位LC-4,采用细胞病变(CPE)观察法和中性红实验法,以利巴韦林为阳性对照药,测定LC-4在最大无毒浓度下于Hela细胞中对RSV的预防作用及治疗作用。结果LC-4的半数中毒浓度(TC50)为25.41μg/ml,半数有效浓度(EC50)为2.11μg/ml,治疗指数(TI)为12.04;LC-4在RSV感染Hela细胞2、4、6、8h后给药,与病毒对照组相比CPE程度差异有显著性(P<0.05);且提前2h给药在LC-4浓度为31.25～3.91μg/ml之间时,与病毒对照相比CPE差异有显著性(P<0.05)。结论LC-4在体外对RSV复制有明显的预防作用及治疗作用。
Objective To study the extraction and isolation of effective fraction of forsythia (LC-4) and its anti-respiratory syncytial virus (RSV) activity in vitro. Methods Forsythia suspensa aqueous extract was subjected to water extraction and alcohol precipitation, macroporous adsorption resin column chromatography and elution to elute and separate the effective fraction of forsythia suspensa antigens LC-4. The cells were treated with cytopathic effect (CPE) The method of sexual redness, taking ribavirin as a positive control drug, determined the preventive effect and therapeutic effect of LC-4 on RSV in Hela cells under the maximum non-toxic concentration. Results The median lethal concentration (LC50) of LC-4 was 25.41μg / ml, the EC50 was 2.11μg / ml and the therapeutic index (TI) was 12.04. LC- (P <0.05). Compared with the control group, the CPE level was significantly different from that of the control group (P <0.05). When the LC-4 concentration was 31.25-3.91μg / ml, CPE difference was significant (P <0.05). Conclusion LC-4 has obvious preventive and therapeutic effects on RSV replication in vitro.