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敞开式离子化质谱(ambient mass spectrometry,AMS)是近年来新兴的一种无需或者只需很少样品前处理步骤,在敞开的大气压环境中可直接对物体表面物质进行离子化的质谱分析技术。本实验室基于敞开式离子源实时直接分析(direct analysis in real time,DART),进行了多方面的研究。主要包括三个方面:第一,应用研究,将简单的样品前处理技术和DART-MS结合,建立了有针对性的快速检测方法;第二,实现正相液相色谱(normal phase liquid chromatography,NPLC)和DART-MS的在线联用,解决了NPLC-MS联用中正相色谱流动相不利于样品离子化的问题,并利用该方法对NPLC分离的手性化合物进行了有效检测;第三,构建了一套新的AMS装置—等离子体辅助多波长激光解吸离子化质谱(plasma assisted multiwavelength laser desorption ioniza-tion mass spectrometry,PAMLDI-MS)。该装置将激光解吸附和等离子体辅助离子化技术相组合,并且将其与简单的TLC快速分离结合,对一些小分子混合物进行了分离检测;实验室还借助简单的铅笔芯中石墨涂覆的滤纸作为PAMLDI-MS的进样基底,发现石墨涂覆的滤纸对于待测样品有很好的基质辅助信号增强作用。
In recent years, open mass spectrometry (AMS) is a new mass spectrometry technique that can directly ionize the surface of an object under open atmospheric pressure without or with few sample preparation steps. Our lab has conducted various studies based on direct analysis in real time (DART). Mainly includes three aspects: First, the application of research, the simple sample preparation technology and DART-MS combined to establish a targeted rapid detection method; second, to achieve normal phase liquid chromatography (normal phase liquid chromatography, NPLC) and DART-MS to solve the problem that NPLC-MS combined with normal-phase mobile phase is not conducive to sample ionization, and the chiral compounds isolated by NPLC were effectively detected by this method. Thirdly, A new AMS device, plasma assisted multiwavelength laser desorption ionization mass spectrometry (PAMLDI-MS), was constructed. The device combines laser desorption with plasma-assisted ionization and combines it with a simple TLC rapid separation to separate and detect some small molecule mixtures. The lab also uses a simple graphite-coated Filter paper As the injection substrate for PAMLDI-MS, graphite coated filter paper was found to have a good matrix-assisted signal enhancement of the sample to be tested.