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目的 观察趋化因子RANTES (“regulateduponactivationnormalTcellexpressedandsecreted”)在慢性移植物抗宿主病 (GVHD)狼疮样小鼠肾组织中的表达及泼尼松的调控作用。方法 GVHD狼疮样小鼠模型参照有关文献建立。按随机设计原则将模型动物分两组 :即模型组 (A组 )和泼尼松治疗组 (B组 ) ,另设正常对照组 (C组 ) ,每组均有 8只动物。RANTES的蛋白表达采用免疫组织化学方法 ,mRNA表达采用原位杂交技术检测。结果 ①模型组 (A组 )肾组织中RANTES蛋白表达显著高于正常对照组 (C组 ) (P <0 0 5 ) ,肾小球和肾小管间质中均有表达 ,尤以肾小管 间质表达更为丰富 ;而泼尼松治疗组 (B组 )RANTES在肾小球和肾小管 间质表达均显著减少 (P <0 0 5 )。②A组肾组织中RANTESmRNA表达显著高于C组 (P <0 0 5 ) ,经泼尼松治疗后RANTESmRNA受到明显抑制。③A组中RANTES蛋白和mRNA表达呈正相关 (r =0 6 12 ,P <0 0 5 )。结论 慢性GVHD狼疮样小鼠肾组织中RANTES蛋白和mRNA表达均显著增高 ,泼尼松在改善肾组织病理改变的同时 ,对RANTES表达亦起抑制作用 ,提示RANTES可能参与了狼疮肾炎的发病机制 ,泼尼松抑制RANTES过度表达可能是其在狼疮肾炎中发挥治疗作用的机制之一。
Objective To observe the expression of chemokine RANTES (“regulateduponactivationnormalTcellexpressedandsecreted”) in renal tissue of chronic graft-versus-host-disease (GVHD) lupus-like mice and the regulation of prednisone. Methods GVHD lupus-like mouse model was established with reference to the literature. According to the principle of random design, the model animals were divided into two groups: the model group (group A) and prednisone treatment group (group B), and another normal control group (group C), each group had 8 animals. RANTES protein expression using immunohistochemistry, mRNA expression using in situ hybridization detection. Results ① The expression of RANTES protein in renal tissue of model group (group A) was significantly higher than that of normal control group (group C) (P <0 05). The expression of RANTES protein in glomerulus and tubulointerstitium, The expression of RANTES in the prednisone group (group B) was significantly decreased in the glomerular and tubulointerstitial tissues (P <0.05). ② The expression of RANTES mRNA in group A was significantly higher than that in group C (P <0.05). RANTES mRNA was significantly inhibited after prednisone treatment. (3) There was a positive correlation between RANTES protein and mRNA expression in group A (r = 0 6 12, P 0 05). CONCLUSION: RANTES protein and mRNA expression were significantly increased in the kidney of chronic GVHD-lupus mice. Prednisone could also inhibit the expression of RANTES while improving the pathological changes of renal tissue, suggesting that RANTES may be involved in the pathogenesis of lupus nephritis, Prednisone inhibition of RANTES overexpression may be one of its mechanisms in the treatment of lupus nephritis.