将ENNG配成5％二甲亚砜溶液给昆明种、LACA小鼠灌肠(0.5mg/次/鼠,6次/周,ENNG最大用量分别为45mg,39mg),诱发出分化较好的大肠腺癌、肛门鳞癌。用组化方法对大肠腺癌18种酶活性变化进行了观察。其中LDH、5’-NT、β-Gr活性增高,SDH、CCO、β-GA活性下降;AKP、LAP、G6Pase活性与对照鼠相同(阴性); The ENNG was formulated into 5% dimethyl sulfoxide solution to the Kunming species and the LACA mice were enema (0.5 mg/time/mouse, 6 times/week, the maximum amount of ENNG was 45 mg, 39 mg, respectively), and the well-differentiated large intestine glands were induced. Cancer, anal squamous cell carcinoma. Eighteen enzyme activities of colorectal adenocarcinoma were observed by histochemical methods. The activities of LDH, 5’-NT, and β-Gr increased, and the activities of SDH, CCO, and β-GA decreased. The activities of AKP, LAP, and G6Pase were the same as those of control mice (negative).