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目的探讨荧光定量聚合酶链反应法(PCR)和培养法对解脲支原体(Uu)的检测特点及Uu感染在阴道炎和宫颈炎中的意义。方法对310例阴道炎宫颈炎患者(研究组)和健康检查者(对照组)216例同时进行Uu荧光定量PCR和培养法检测,培养法行药敏检测。结果在研究组中荧光定量PCR和培养法检测Uu的阳性率分别为52.25%和43.87%,对照组分别为24.54%和20.37%,经χ2检验在研究组中2种方法检测Uu的阳性率均显著高于对照组(P<0.01);荧光定量PCR的阳性率显著高于培养法(P<0.01);药物敏感试验结果显示,Uu培养法阳性者对喹诺酮类药物的耐药率呈较高水平。结论荧光定量PCR检测Uu的敏感性要高于培养法,但是培养法的药敏试验可及时准确地指导临床选择用药;阴道炎和宫颈炎与Uu感染关系密切,强力霉素、克拉霉素、美满霉素为治疗解脲支原体感染的首选药物。
Objective To investigate the detection characteristics of Uu mycoides (Uu) and the significance of Uu infection in vaginitis and cervicitis by fluorescence quantitative polymerase chain reaction (PCR) and culture methods. Methods A total of 216 cases of vaginitis cervicitis (study group) and health check-up (control group) were detected by Uu fluorescence quantitative PCR and culture method simultaneously. Results In the study group, the positive rate of Uu detected by fluorescence quantitative PCR and culture method were 52.25% and 43.87% respectively, while the control group was 24.54% and 20.37% respectively. The positive rates of Uu detected by χ2 test in the study group were (P <0.01). The positive rate of fluorescence quantitative PCR was significantly higher than that of the culture method (P <0.01). The results of drug sensitivity test showed that the drug resistance rate of quinolones to Uu culture was higher Level. Conclusion The sensitivity of fluorescent quantitative PCR for detecting Uu is higher than that of culture method. However, the sensitivity test of culture method can guide the clinical choice of drug timely and accurately. Vaginitis and cervicitis are closely related to Uu infection. Doxycycline, clarithromycin, Minocycline is the drug of choice for the treatment of Ureaplasma urealyticum infection.