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以东北红豆杉(Taxus cuspidata)3a生实生苗和扦插苗为实验材料,研究干旱和复水后实生苗和扦插苗的叶绿素荧光和抗氧化酶活性变化规律,为更好地培育东北红豆杉资源提供理论依据。研究结果表明,实生苗对于干旱适应性强于扦插苗。在干旱处理过程中,随着干旱条件加剧,实生苗和扦插苗初始荧光Fo和非光化学猝灭系数NPQ均表现出升高趋势,扦插苗Fo升幅较快,而实生苗NPQ升幅较大。与实生苗相比,扦插苗的PSⅡ最大量子效率(Fv/Fm),PSⅡ实际光化学量子效率(ФPSⅡ)和光合电子传递速率(ETR)表现出较大幅度的降低,说明扦插苗对干旱胁迫较敏感。但复水后,实生苗和3a生扦插苗荧光参数均恢复到正常水平。干旱胁迫条件下,实生苗和扦插苗抗氧化酶SOD、CAT和POD均表现出升高。
In order to better cultivate Taxus cuspidata 3a seedlings and cutting seedlings, the changes of chlorophyll fluorescence and antioxidant enzyme activities of seedlings and cutting seedlings after drought and rewatering were studied. Provide a theoretical basis. The results showed that the seedlings have stronger adaptability to drought than cutting seedlings. In the process of drought treatment, the initial fluorescence Fo and non-photochemical quenching coefficient NPQ of seedlings and cuttings showed an increasing trend with the aggravating of drought conditions. Fo of cutting seedlings increased rapidly while the NPQ of seedlings increased significantly. Compared with seedlings, the PSⅡ maximum quantum yield (Fv / Fm), PSⅡ actual photochemical quantum efficiency (ФPSⅡ) and photosynthetic electron transfer rate (ETR) showed a significant reduction, indicating that cutting seedlings to drought stress more sensitive. However, after rewatering, the fluorescence parameters of seedlings and 3a seedlings returned to normal levels. Under drought stress, the antioxidant enzymes SOD, CAT and POD of seedlings and cutting seedlings all showed an increase.