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作者选择免疫后具有抗D 同种抗体的病人,取外周血单核细胞经EBV 病毒转化与P_3X_(63)Ag~(?)鼠骨髓瘤细胞,在聚乙醇(PEG400)的作用下,融合成杂交瘤细胞,经培养后上清液用木瓜酶处理的红细胞做微量凝集试验,筛选出分泌JK~(?)单克隆抗体杂交瘤细胞,连续培养1年后控制产品的稳定性,应用酶处理的红细胞悬液和低离子强度(LISS)红细胞悬液做血清学检查,应用酶联免疫测定杂交瘤上清液免疫球蛋白的分类和定量。
The authors selected patients with anti-D allo-antibodies after immunization. Peripheral blood mononuclear cells were transformed with P_3X_ (63) Ag ~ (~) myeloma cells by EBV virus and fused with PEG400 Hybridoma cells, the supernatant after culture by papain-treated erythrocytes do microagglutination test to screen out the secretion of JK? (?) Monoclonal antibody hybridoma cells, continuous culture for 1 year after the control of product stability, the application of enzyme treatment (LISS) erythrocyte suspension for serological examination and the classification and quantification of hybridoma supernatant immunoglobulins by enzyme-linked immunosorbent assay.