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目的探讨异基因造血干细胞移植时,供体 CD8~+T 细胞致受体血管内皮细胞的凋亡效应及其信号机制。方法采用密度梯度法分离人周围血单个核细胞,用 CD8~+T 细胞亚群阴性分选柱试剂盒分选出 CD8~+T 细胞。将人脐静脉内皮细胞株(HUVEC)分为4组:低剂量 TNF 处理混合组和
Objective To investigate the apoptotic effect of donor CD8 ~ + T cells on vascular endothelial cells and its signal mechanism during allogeneic hematopoietic stem cell transplantation. Methods Human peripheral blood mononuclear cells were isolated by density gradient method and CD8 + T cells were sorted by CD8 + T cell subsets negative sorting kit. Human umbilical vein endothelial cell line (HUVEC) were divided into 4 groups: low-dose TNF-treated mixed group and