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目的检测内蒙地区结直肠癌K-ras基因突变情况,并结合临床病理资料加以分析。方法提取15例结直肠癌患者结直肠癌手术切除标本组织的DNA,对产物进行基因序列癌组织的DNA聚合酶链反应(PCR)扩增、DNA直接测序分析。结果 K-ras基因突变率为0%,几种分化型的结直肠癌均未发现K-ras基因突变类型,包括12密码子(GGT)、13密码子(GGC)。结论我院结直肠癌患者k-ras基因突变率为0%,转移性结直肠癌患者原发肿瘤与转移灶肿瘤k-ras基因型均相同;结直肠癌患者k-ras基因突变与否与年龄、性别、肿瘤浸润深度、肿瘤组织学类型无关。
Objective To detect the mutation of K-ras gene in colorectal cancer in Inner Mongolia and to analyze the clinicopathological data. Methods DNA from 15 patients with colorectal cancer and 15 patients with colorectal cancer was collected. DNA was amplified by polymerase chain reaction (PCR) and sequenced. Results The mutation rate of K-ras gene was 0%. There was no K-ras gene mutation type, including 12 codons (GGT) and 13 codons (GGC) in several differentiated colorectal cancers. Conclusions The mutation rate of k-ras gene in colorectal cancer patients is 0% in our hospital. The k-ras gene mutations of primary tumor and metastatic tumor are the same in metastatic colorectal cancer patients. Age, gender, tumor invasion depth, tumor histological type has nothing to do.