随机扩增多态性DNA(RAPD)是目前常用的DNA指纹图谱技术。采用改进的“ROSE法”提取DNA ,并建立了重复性较好的RAPD标准分析条件。在此标准条件下 ,检测了烟草 10个品种材料基因组的多态性。经 10个引物扩增 ,在得到的 53条扩增带中 ,有 10个片段为 10个材料所共有 ,多态性达81.1%。其中引物P7的扩增图谱 ,各品种间可以互相区别 ,差异明显 ,可用作烟草 10个品种鉴定的DNA指纹图谱 Random amplified polymorphic DNA (RAPD) is the most commonly used DNA fingerprinting technique. The improved “ROSE method” was used to extract DNA, and the standard RAPD analysis conditions were established. Under this standard condition, the genomic polymorphism of 10 varieties of tobacco was tested. After 10 primers were amplified, of the 53 amplified bands obtained, 10 fragments shared 10 materials with a polymorphism of 81.1%. Among them, the amplification pattern of primer P7 could be distinguished from each other, and the difference was obvious. It could be used as DNA fingerprinting of 10 varieties of tobacco