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目的分析乙型肝炎表面抗原(HBsAg)和丙型肝炎病毒抗体(抗-HCV)酶联免疫检测与血液病毒核酸筛查技术的关系。方法选择13085个血样标本,经HBsAg与抗-HCV酶联免疫检测,对确认阳性及阴性的血样标本进行血液病毒核酸方面的筛查,分析HBs Ag与抗-HCV酶联免疫检测与血液病毒核酸筛查技术之间的关系。结果经HBsAg酶联检测显示,13085个血样标本中阳性219个,约占1.67%,分别经过国产或进口试剂检测分析,其一致率为99.55%;经抗-HCV酶联免疫检测显示,13085个血液样本中阳性者108个,约占0.83%,分别经过国产或进口试剂检测分析,其一致率为95.37%;再次确认HBsAg结果显示219个中阳性者198个,阴性者21个,分别经过国产或进口试剂检测分析,其一致率为90.41%;经抗-HCV再次检测确认108个中阳性者57个、阴性者44个、可疑结果7个,分别经过国产或进口检测试剂分析,其一致率为59.26%。将血液标本经核酸筛查技术测定乙型肝炎病毒核酸(HBV DNA)显示结果阳性者195个,阴性17个,而丙型肝炎病毒核酸(HCV RNA)测定结果显示阳性者72个,阴性者36个;合并计算这两种因素,经酶联免疫法测定及再次确认测定灵敏度为97.26%,其特异性计算为94.44%。结论经过HBs Ag及抗-HCV酶联免疫检测,其检测结果显示灵敏度及特异度均良好,但因试剂盒有所不同而出现误检的情况,但血液核酸筛查技术能够有效避免误检情况出现,促进检出率提高。
Objective To analyze the relationship between hepatitis B virus surface antigen (HBsAg) and Hepatitis C virus (anti-HCV) enzyme-linked immunosorbent assay and blood viral nucleic acid screening. Methods A total of 13085 samples of blood samples were selected and tested by HBsAg and anti-HCV enzyme-linked immunosorbent assay (ELISA) to screen for blood viral nucleic acid in positive and negative blood samples. The relationship between HBs Ag, anti-HCV enzyme immunoassay and blood viral nucleic acid The relationship between screening techniques. Results The results of HBsAg ELISA showed that there were 219 positive samples in 13085 blood samples, accounting for 1.67% of them, respectively. The coincidence rate was 99.55% after detection by domestic or imported reagents. The anti-HCV ELISA showed that 13085 There were 108 positive samples in the blood samples, accounting for 0.83%. The detection rate was 95.37% after domestic or imported reagents were tested respectively. The results of HBsAg re-confirmation showed that 219 positive samples were 198 and negative ones were 21 Or imported reagent detection and analysis, the agreement rate was 90.41%; retest by anti-HCV confirmed 108 positive in 57, negative 44, the suspicious results of 7, respectively, after domestic or imported testing reagents analysis, the consistency rate 59.26%. Blood samples were detected by nucleic acid screening of hepatitis B virus nucleic acid (HBV DNA) showed positive 195, negative 17, and hepatitis C virus RNA (HCV RNA) test results showed that positive 72, negative 36 A; combined calculation of these two factors, the enzyme-linked immunosorbent assay and re-confirm the determination of sensitivity was 97.26%, the specificity was 94.44%. Conclusion The detection results of HBs Ag and anti-HCV ELISA showed that the sensitivity and specificity were good, but the false positives occurred due to different kits. However, the blood nucleic acid screening technology could effectively avoid the false positives Appear to promote the detection rate increased.