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目的:探讨黄芪皂苷(AST)抑制HEK293sw细胞-淀粉样肽(Aβ)生成的作用机制。方法:采用HEK293sw细胞,常规培养后加入不同浓度(0,0.1,1和10μmo.lL-1)AST处理,荧光法测定β-分泌酶活力,并用Western blot和RT-PCR技术检测淀粉样前体蛋白(APP)和β-位点APP切割酶1(BACE1)的表达。结果:在HEK293sw细胞模型上,AST终浓度1和10μmol.L-1显著降低β-分泌酶活力。1和10μmol.L-1AST对APP的蛋白和mRNA表达无影响,但对BACE1的蛋白水平都有降低作用,同时也显著降低mRNA的水平。结论:AST可能通过抑制β-分泌酶而降低Aβ的产生。
Objective: To investigate the mechanism of astragalosides (AST) inhibiting the production of amyloid peptide (Aβ) in HEK293sw cells. METHODS: HEK293sw cells were treated with different concentrations (0, 0.1, 1 and 10 μmol·lL-1) of AST after routine culture. β-secretase activity was assayed by fluorescence and amyloid precursors were detected by Western blot and RT-PCR. Protein (APP) and β-site expression of APP cleavase 1 (BACE1). RESULTS: On the HEK293sw cell model, AST final concentrations of 1 and 10 [mu]mol.L-1 significantly decreased [beta]-secretase activity. 1 and 10 μmol.L-1 AST had no effect on the protein and mRNA expression of APP, but had a decreased effect on the protein level of BACE1, and also significantly decreased the mRNA level. Conclusion: AST may reduce Aβ production by inhibiting β-secretase.