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[目的]分析成都平原主养草鱼、搭配鲫鱼池塘底泥中的细菌群落结构。[方法]通过构建细菌16S rRNA基因克隆文库,对阳性克隆子进行多样性分析和构建系统发育树。[结果]依据97%序列相似性划分OUT,所构建的文库共获得171个克隆子,并划分为14个OTUs(操作分类单元),其多样性指数Shannon(H)和优势度指数Simpson(D)分别为2.12和7.15,丰富度指数SChao1和SACE均为16。池塘底泥中主要的细菌类群为:梭杆菌门(24%)、变形菌门(55%)、浮霉菌门(10.5%)、绿弯菌门(5.9%)、拟杆菌门(2.3%)、放线菌门(1.8%)和厚壁菌门(0.6%)。[结论]Cetobacterium、Prolixibacter、Rhodocyclus、Candidate、Planctomycetacia、Thiocapsa、Desulfomicrobium和Sterolibacterium等属菌在脱氮除磷、降低硫化物、降解有机质等发挥重要作用。池塘底泥既是水生态系统修复功能菌的资源库,但也存在着Xanthomonas等致病性和功能未知的细菌库。
[Objective] The research aimed to analyze the main grass carp in Chengdu Plain and the bacterial community structure in the sediment of crucian carp pond. [Method] By constructing the bacterial 16S rRNA gene clone library, the diversity of positive clones was analyzed and the phylogenetic tree was constructed. [Result] Based on 97% sequence similarity, 171 clones were obtained and divided into 14 OTUs (operating taxa). Shannon (H) and Simpson (D) ) Were 2.12 and 7.15, respectively. The index of richness SChao1 and SACE were both 16. The main bacterial groups in pond sediment are Fusobacterium (24%), Proteobacteria (55%), Botrytis (10.5%), Meloidogyne (5.9%), Bacteroidetes (2.3%), , Actinomyces (1.8%) and Fusarium (0.6%). [Conclusion] Cetobacterium, Prolixibacter, Rhodocyclus, Candidate, Planctomycetacia, Thiocapsa, Desulfomicrobium and Sterolibacterium all play important roles in nitrogen and phosphorus removal, sulfide reduction and organic matter degradation. Pond sediment is not only a repository for water-ecosystem restoration fungi, but also a pathogenic and function-unknown bacteria pool such as Xanthomonas.