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[Objectives] For qualitative and quantitative investigation of Astragalus saponin semi-finished products,to establish the Astragalus content determination method,determine the content of Astragalus saponins in semi-finished products,and provide full guarantee for the followup preparation and product quality control.[Methods]With Astragaloside A as reference substance,TLC method was used for qualitative determination of the main components in semi-finished products of Astragalus saponin. Using colorimetry,the content was determined,and with sulfuric acid as chromogenic reagent,the wavelength was measured to be 320 nm.[Results]When the concentration of Astragaloside A was 1.04-8.32 μg·ml~(-1),there was a linear correlation between absorbance and concentration,and precision,stability and reproducibility were in line with standards. The content of Astragaloside A in semi-finished products of Astragalus saponin was measured to be 50- 60%. [Conclusions]The sulfuric acid chromogenic method could be used to determine the content of Astragaloside A in semi-finished products of Astragalus saponins,and it had good precision,stability and reproducibility.
[Objectives] For qualitative and quantitative investigation of Astragalus saponin semi-finished products, to establish the Astragalus content-determining method, determine the content of Astragalus saponins in semi-finished products, and provide full guarantee for the followup preparation and product quality control. [ Methods] With Astragaloside A as reference substance, TLC method was used for qualitative determination of the main components in semi-finished products of Astragalus saponin. Using colorimetry, the content was determined, and with sulfuric acid as chromogenic reagent, the wavelength was measured to be 320 nm. [Results] When the concentration of Astragaloside A was 1.04-8.32 μg · ml -1, there was a linear correlation between absorbance and concentration, and precision, stability and reproducibility were in line with standards. The content of Astragaloside A in semi-finished products of Astragalus saponin was measured to be 50- 60% [Conclusions] The sulfuric acid chromogenic method could be used to determine the content of Astragaloside A in semi-finished products of Astragalus saponins, and it had good good, stability and reproducibility.