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研究核因子NF-κB在低氧性肺动脉高压大鼠肺组织表达的变化,探讨NF-κB在低氧性肺动脉高压发病机制中的作用。将20只SD大鼠随机分为正常对照组、低氧组,以常压低氧3周复制肺动脉高压模型,测量大鼠右心室Rright Ventricle(RV)和左心室+室间隔Left ventricle+septum(LV+S)重量,以RV/(LV+S)代表其右心室肥厚指数;对大鼠肺组织切片采用HE染色,经图像分析技术测定大鼠肺小动脉管壁厚度占血管外径的百分比Wall thickness/external diameter(WT%)和管壁面积占血管总面积的百分比Wall area/total vascular area(WA%)反映其肺血管重建情况。对大鼠肺组织切片采用免疫组化法观察2组大鼠肺组织NF-κB表达的变化。结果表明:1)低氧组大鼠RV/(LV+S)为(33.11±0.95)%、WT%为(22.36±2.99)%、WA%为(69.14±5.38)%,正常对照组RV/(LV+S)为(24.48±0.56)%、WT%为(13.30±2.77)%、WA%为(46.75±6.54)%,两组之间的差异有显著性(P均<0.01)。2)低氧组细支气管上皮细胞NF-κB核染色阳性细胞百分比为(29.11±1.12)%,与正常组(12.23±1.08)%比较,差异有显著性(P<0.01)。NF-κB在低氧性肺动脉高压大鼠肺组织表达增加,可能为低氧性肺动脉高压作用机制之一。
To investigate the expression of nuclear factor-kappa B (NF-κB) in the lung tissue of hypoxic pulmonary hypertensive rats and to explore the role of NF-κB in the pathogenesis of hypoxic pulmonary hypertension. 20 SD rats were randomly divided into normal control group and hypoxia group. The model of pulmonary hypertension was established by normobaric hypoxia for 3 weeks. Rright Ventricle (RV) and left ventricular + septum LV + S) weight, RV / (LV + S) on behalf of the right ventricular hypertrophy index; rat lung tissue sections were stained with HE, by image analysis of rat pulmonary arterioles wall thickness as a percentage of vascular diameter Wall thickness / external diameter (WT%) and wall area as a percentage of the total area of the vessel Wall area / total vascular area (WA%) reflects its pulmonary vascular remodeling. The changes of NF-κB in the lung tissue of the two groups were observed by immunohistochemistry on the rat lung tissue sections. The results showed that: 1) The RV / (LV + S) in the hypoxic group was (33.11 ± 0.95)%, the WT% was (22.36 ± 2.99)%, the WA% was (69.14 ± 5.38) (24.48 ± 0.56)% for LV + S, (13.30 ± 2.77)% for WT and (46.75 ± 6.54)% for WA. There was significant difference between the two groups (all P <0.01). (2) The percentage of NF-κB nuclear staining positive cells in hypoxic group was (29.11 ± 1.12)%, which was significantly different from that in normal group (12.23 ± 1.08)% (P <0.01). The expression of NF-κB in the lung tissue of hypoxic pulmonary hypertension rats may be one of the mechanisms of hypoxic pulmonary hypertension.