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通过摇瓶培养，从收集的四支金针菇菌株中筛选出一支较适合于悬浮培养的菌株－ＷＪ．研究了锌盐对菌株ＷＪ生长的影响及ＷＪ对锌盐的吸收，得出１０００ｍｇ／ＬＺｎＳＯ＿４为较佳添加锌浓度。７００Ｌ发酵罐放大实验：菌丝体得率为０．９２％，有机锌含量为４．７８ｍｇ／ｇ．发酵液中的菌丝体经过滤、洗涤、抽提、浓缩等工序加工制成口服液并对样品进行了检测。产品经小鼠免疫功能实验及益智实验，实验组与对照组有显著差异。
By shake flask culture, from the collected four Flammulina strains screened a more suitable strain for suspension culture-WJ. The effects of zinc salts on the growth of strain WJ and the absorption of zinc salts by WJ were studied. It is concluded that zinc concentration of 1000 mg / L ZnSO 4 is the best. 700L fermenter amplification experiments: mycelium yield of 0.92%, organic zinc content of 4.78mg / g. The mycelium in the fermentation broth was processed into oral solution by filtration, washing, extraction and concentration, and the samples were tested. Products by immune function experiments and puzzle experiments in mice, the experimental group and the control group were significantly different.