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目的探讨血凝反应法检测黄毛鼠对抗凝血杀鼠剂抗性的可行性。方法以杀鼠灵(warfarin)抗药性区分剂量(10mg/kg)单次灌胃处理结合致死期食毒抗性检测法(LFP)筛选出黄毛鼠敏感种群和抗性种群,在不同时间段采集试鼠血浆,通过检测试鼠血浆的凝血酶活度(PCA)建立黄毛鼠凝血反应标准曲线,并分析抗性区分剂量处理后抗性个体与敏感个体PCA的变化差异。结果建立了黄毛鼠的凝血反应标准曲线:INR(y)=34.984/x+0.688(x=PCA)(R2=0.992);以10mg/kg为区分剂量单次灌胃处理后,抗药性黄毛鼠个体的PCA虽有所下降,但可在2～3d内恢复到正常凝血水平的17%左右;敏感个体的PCA可下降到很低,且不能恢复。结论证实了以血凝反应法检测黄毛鼠对抗凝血剂抗药性的可行性:杀鼠灵10mg/kg为区分剂量单次灌胃处理4d后,以PCA=16.5(或INR=4.4)作为阈值来区分黄毛鼠抗药性与敏感性个体,是准确、简便的抗药性判定方法。
Objective To investigate the feasibility of using hemagglutination assay to detect the resistance of rhesus rat to anticoagulant rodenticides. Methods The susceptible and susceptible populations of rhesus were screened by a single gavage at a dosage of warfarin resistant (10 mg / kg) combined with lethal phase edible toxin resistance assay (LFP). At different time periods The plasma of test rats was collected. The standard curve of thoracic coagulation reaction was established by detecting the plasma thrombin activity (PCA) in test rats. The difference of PCA between resistant and susceptible individuals was analyzed. Results The standard curve of coagulation reaction of Rattus norvegicus was established: INR (y) = 34.984 / x + 0.688 (x = PCA) (R2 = 0.992); after single intragastric administration at dose of 10mg / Although the PCA of the hairless rat has decreased, it can recover to about 17% of the normal blood coagulation level within 2 ~ 3d. The PCA of sensitive individuals can be reduced to very low and can not be recovered. CONCLUSIONS: The feasibility of using hemagglutination assay to detect the resistance of the rhesus rat to anticoagulants was confirmed. After the single dose of warfarin 10 mg / kg was administered for 4 days, PCA = 16.5 (or INR = 4.4) To distinguish between rodent resistance and susceptibility of individuals, is accurate and easy way to determine resistance.