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目的建立口腔黏膜组织癌变趋势的判别方法,为临床肿瘤或疑似癌变组织的早期诊断奠定研究基础。方法采用RT-PCR技术检测口腔正常组织、口腔黏膜白斑病组织(oral leukoplakia,OLK)和口腔鳞状细胞癌(oralsquamous cell carcinoma,OSCC)组织的肿瘤相关基因(NF-1,ACP-2,BCL-2,CLK-3,FKBP-8,SOCS-3,XRCC-1,CTNNB-1,GDF-15)表达水平,采用Fisher判别分析建立口腔黏膜组织癌变趋势大小的基因诊断方法,采用Cross-validated(a)法对本判别方法进行评价。结果 ACP-2,BCL-2及SOCS-3 3个基因的表达水平在3种组织间逐渐降低(P<0.05),而NF-1,CLK-3,FKBP-8,XRCC-1,CTNNB-1及GDF-15 6个基因的表达水平在3种组织间逐渐升高(P<0.05);口腔黏膜组织癌变趋势的判别公式:Y1=-16.811+0.477 XNF-1+0.540 XACP-2-0.543 XCTNNB-1-0.089 XSOCS-3;Y2=-35.832+0.073 XNF-1-0.074 XACP-2+0.306 XCTNNB-1+0.191 XSOCS-3;判别界值>8.6513可判定为无癌变,0.1117～8.6513为轻度癌变组织,<0.1117为癌变组织;采用Cross-validated(a)法进行评价,总判别符合率为100%;交互判别符合率为100%,灵敏度为100%,特异度为100%。结论本研究建立了口腔黏膜组织癌变趋势判别公式,有助于其基因诊断方法的建立。
OBJECTIVE: To establish a method for determining the cancerous tendency of oral mucosa, and lay the foundation for the early diagnosis of clinical tumor or suspected cancerous tissue. Methods RT-PCR was used to detect the expression of tumor-associated genes (NF-1, ACP-2, BCL) in oral leukoplakia (OLK) and oral squamous cell carcinoma (OSCC) 2, CLK-3, FKBP-8, SOCS-3, XRCC-1, CTNNB-1 and GDF-15) were detected by Fisher discriminant analysis. (a) Act to evaluate this discriminant method. Results The expression of ACP-2, BCL-2 and SOCS-3 decreased gradually among the three tissues (P <0.05) 1 and GDF-15 in three kinds of tissues increased gradually (P <0.05). The discriminant formula of cancerous tendency in oral mucosa was Y1 = -16.811 + 0.477 XNF-1 + 0.540 XACP-2-0.543 XCTNNB-1-0.089 XSOCS-3; Y2 = -35.832 + 0.073 XNF-1-0.074 XACP-2 + 0.306 XCTNNB-1 + 0.191 XSOCS-3; discriminant cut-off value> 8.6513 was judged as cancerous, 0.1117-8.6513 was light Degree of cancerous tissue, <0.1117 for the cancerous tissue; using Cross-validated (a) method of evaluation, the total discriminant compliance rate was 100%; interactive discriminant compliance rate was 100%, the sensitivity was 100%, the specificity was 100%. Conclusion This study established a discriminant formula of carcinogenesis tendency in oral mucosa, which is helpful to establish the method of gene diagnosis.