The effects of biosolids,ZnO,and ZnO/biosolids on soil microorganism and the environmental fate of coexisting racemic-quizalofop-ethyl (rac-QE) were investigated.Microbial biomass carbon in native soil,soil/biosolids decreased by 62％ and 52％ in the presence of ZnO (2％,weight ratio).The soil bacterial community structure differed significantly among native soil,soil/biosolids,soil/ZnO,and soil/biosolids/ZnO based on a principal co-ordinate analysis (PCoA) of OTUs and one-way ANOVA test of bacterial genera.Chemical transformation caused by ZnO only contributed 4％ and 3％ of the overall transformation of R-quizalofop-ethyl (R-QE) and S-quizalofop-ethyl (S-QE) in soil/ZnO.The inhibition effect of ZnO on the initial transformation rate of R-QE (rR-QE) and S-QE (rR-QE) in soil only observed when enantiomer concentration was larger than 10 mg/kg.Biosolids embedded with ZnO (biosolids/ZnO) caused a 17％-42％ and 22％-38％ decrease of rR-QE and rS-QE,although rR-QE and rS-QE increased by 0％-17％ and 22％-58％ by the addition of biosolids.The results also demonstrated that the effects of biosolids on agricultural soil microorganism and enantioselective transformation of chiral pesticide was altered by the embedded nanoparticles.