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从组织形态学角度观察脉冲电场和直流电场作用后皮肤结构的变化 ,以阐明这两种技术的差别。将人皮和蛇皮置于 Valia- Chien双室扩散池 ,用电穿孔脉冲电场 (脉幅 380 V,脉率 4ppm,脉宽 5 .5 ms,脉冲数 10 0个 ,电容量 2 2 μF)和离子导入直流电流 (0 .5 m A/cm2 )作用于这些皮肤 ,用荧光显微镜观察被动扩散 (对照 )、离子导入和电穿孔三组皮肤的结构变化 ,荧光素为 FITC。结果表明 ,对于人皮被动扩散见有少量 15～ 30 μm边缘粗糙的高亮荧光斑点 ;离子导入见高亮荧光斑点边缘变得光滑 ,尺度不变 ;电穿孔的高亮荧光斑点扩大至 80～ 90 μm,边缘光滑。对于蛇皮被动扩散和离子导入都未观察到高亮荧光斑点 ,但在电穿孔观察到 2 5～ 30 μm的高亮荧光斑点。提示脉冲电场可以诱致皮肤电穿孔 ,产生药物经皮渗透的新途径
From the perspective of histomorphology, the changes of skin structure after pulsed electric field and direct electric field were observed to clarify the difference between the two techniques. The human skin and the snake skin were placed in a Valia-Chien double-chamber diffusion cell and electroporated with a pulsed electric field (pulse width 380 V, pulse rate 4 ppm, pulse width 5.5 ms, pulse number 10 0, capacitance 2 2 μF) (0. 5 m A / cm2) were applied to the skin. Fluorescence microscopy was used to observe the structural changes of passive diffusion (control), iontophoresis and electroporation. The fluorescein was FITC. The results showed that a small amount of bright fluorescent spots with rough edges of 15-30 μm were observed for the passive diffusion of human skin. The edges of the bright fluorescent spots became smooth and the scale was unchanged by iontophoresis. The bright fluorescent spots of electroporation expanded to 80 ~ 90 μm, smooth edges. No hyperintense fluorescent spots were observed for passive diffusion and iontophoresis of snakeskin, but bright fluorescent spots of 25-30 μm were observed in electroporation. Tip pulse electric field can induce skin electroporation, resulting in a new way of drug transdermal penetration