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目的:探讨在大鼠模拟高强度一次性力竭、负荷累积军训肾损伤两种模型中,肾组织肾损伤分子-1(KIM-1)、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)表达的变化及其意义。方法:(1)雄性12周龄SD大鼠58只:模拟高强度军事一次性力竭训练尿检(TU)组10只、血检(TS)组48只,构建高强度一次性力竭军训肾损伤模型。留取训练前后不同时间TU组尿样,TS组血清、肾组织样本。(2)雄性4周龄SD大鼠56只:模拟高强度军事负荷累积训练尿检对照(CUC)组8只、尿检训练(CUT)组8只、血检对照(CSC)组20只、血检训练组(CST)组20只,构建高强度负荷累积军训肾损伤模型。留取每2周末次训练后CUC、CUT组24 h尿液;每2周处死CSC、CST组各10只,取血、肾组织。(3)检测血、尿KIM-1、NGAL浓度。免疫组化法测肾组织KIM-1、NGAL表达,计算阳性染色单位累积光密度值(IOD)。结果:TS组大鼠训练后肾组织见KIM-1、NGAL表达明显增强。6 h KIM-1、NGAL的IOD最高。肾组织KIM-1、NGAL表达的强度与尿液KIM-1、NGAL水平正相关。CST组大鼠在训练第2周,肾组织KIM-1、NGAL的表达即强于同期对照组。随训练强度、时间增加,IOD值逐渐增加,与尿液KIM-1、NGAL、肾小管半定量积分正相关。结论:尿中KIM-1、NGAL在反映高强度军训肾损伤中具有良好的敏感性。
OBJECTIVE: To investigate the effects of KIM-1 on nephropathy, neutrophil gelatinase-associated lipocalin (NGAL) in rats with simulated one-time exhaustive exhaustion and military training kidney injury in rats. ) Expression and its significance. Methods: (1) Fifty-eight male Sprague-Dawley rats of 12 weeks old were randomly divided into four groups: 10 rats in high intensity military training one time exhaustive urine test (TU) and 48 children in blood test (TS) Damage model. TU urine samples taken before and after training at different times, TS group serum and kidney tissue samples. (2) 56 male 4-week-old SD rats: 8 rats in CUC group, 8 in urine test group (CUT), 20 in blood test control group (CSC) Twenty rats in training group (CST) were enrolled in this study. The urine of CUC and CUT group was collected 24 hours after training every 2 weeks; 10 cases of CSC and CST group were sacrificed every 2 weeks, blood and kidney tissues were collected. (3) blood, urine KIM-1, NGAL concentration. Immunohistochemistry was used to measure the expression of KIM-1 and NGAL in renal tissue, and the cumulative optical density (IOD) of positive staining units was calculated. Results: After training, rats in TS group showed KIM-1 in renal tissue, and the expression of NGAL was significantly increased. 6 h KIM-1, NGAL the highest IOD. The intensity of KIM-1 and NGAL expression in renal tissue was positively correlated with urine KIM-1 and NGAL levels. In the second week of training, the expression of KIM-1 and NGAL in renal tissue of CST group was stronger than that of the control group. With the training intensity and time increasing, the IOD value increased gradually, positively correlated with the semi-quantitative integral of urinary KIM-1, NGAL and tubular. Conclusion: Urinary KIM-1 and NGAL have good sensitivity in reflecting high-intensity military training of kidney injury.