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目的 :研究转基因小鼠高表达的FasL对Sertoli细胞在睾丸局部感染时的免疫调节作用的影响。方法 :将溶脲脲原体 (UU)分别注入FasL转基因及野生型小鼠膀胱 ,模拟上行性感染的途径。分别在感染后 1、2和 3wk处死小鼠 ,分离睾丸组织观察其病理变化 ,并用免疫组化染色法比较UU感染前后 ,Ser toli细胞上FasL和TGF β、IL 1α、IL 6的表达及分泌格局的差异。从野生型小鼠睾丸组织中分离高纯度的Sertoli细胞 ,与未感染UU的对照组相比 ,观察UU感染后FasL+ Sertoli细胞介导Fas+ Jurkat细胞的凋亡能力的变化。结果 :UU感染组的转基因小鼠 ,睾丸组织发生的病理改变比野生型更为明显 ;两种小鼠感染后Sertoli细胞分泌的调节因子变化格局不同 ;感染后Sertoli细胞对Jurkat细胞的杀伤能力增强。结论 :在抗感染免疫中 ,转基因表达的FasL可影响Sertoli细胞分泌细胞因子的格局 ,进而影响睾丸局部的免疫平衡。过高表达的FasL对机体的抗感染应答并非一定有利。
OBJECTIVE: To study the effect of FasL overexpression in transgenic mice on the immunoregulatory effects of Sertoli cells on local testis infection. Methods: Uroaplasma urealyticum (UU) was injected into the bladder of FasL transgenic and wild-type mice respectively to simulate the pathways of ascending infection. The mice were sacrificed at 1, 2 and 3wk after infection respectively, and the pathological changes of testis were observed. The expression and secretion of FasL, TGFβ, IL-1α and IL-6 on Ser toli cells before and after UU infection were compared by immunohistochemical staining Differences in pattern. Sertoli cells were isolated from testicular tissue of wild-type mice, and compared with control group without UU, the apoptosis of Fas + Jurkat cells was observed after Fas / Sertoli cells were infected with UU. Results: The pathological changes in transgenic mice and testis tissues of UU infected group were more obvious than those in wild type. The changes of regulatory factors secreted by Sertoli cells were different between the two groups. The ability of Sertoli cells to kill Jurkat cells was enhanced after infection . CONCLUSIONS: In anti-infective immunity, FasL expressed by transgene can affect the pattern of cytokines secreted by Sertoli cells and further affect the immune balance in testis. Over-expression of FasL on the body’s anti-infective response is not necessarily beneficial.