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目的:研究熟地黄醇提物毛蕊花糖苷在正常大鼠和血虚模型大鼠体内药动学差异。方法:皮下注射乙酰苯肼联合环磷酰胺复制大鼠血虚模型,熟地黄醇提物灌胃给药,采用高效液相色谱法测定不同时间点大鼠血浆中蕊花糖苷的血药浓度,采用3p87软件计算药动学参数。结果:毛蕊花糖苷在该测定方法下线性范围为0.2~80μg/m L,最低检测限和最低定量限分别为0.03、0.12μg/m L;与正常对照组比较,模型组大鼠相应剂量组AUC0-t和AUC0-∞显著增加,平均驻留时间和消除半衰期显著延长。结论:该方法专属性强、灵敏度高,操作简便,可用于检测血虚模型下毛蕊花糖苷的药动学过程。
OBJECTIVE: To study the pharmacokinetics of alcohol extract of Rehmannia glutinosa in vivo in normal rats and blood-deficient rats. Methods: The model of blood deficiency was induced by subcutaneous injection of acetophenhydrazine combined with cyclophosphamide in rats, and the ethanol extract of Rehmannia glutinosae was administered intragastrically. The plasma concentrations of abacus glycosides in rats at different time points were determined by high performance liquid chromatography (HPLC) Pharmacokinetic parameters were calculated using 3p87 software. Results: The linear range of verbascoside was 0.2 ~ 80μg / m L in this method. The minimum and maximum limits of detection were 0.03 and 0.12μg / m L, respectively. Compared with the normal control group, the AUC0 -t and AUC0-∞ significantly increased, mean residence time and elimination half-life significantly prolonged. Conclusion: This method is specific, sensitive and easy to operate. It can be used to detect the pharmacokinetics of verbascoside in blood deficiency model.