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本实验利用空斑形成细胞(简称PEC)测定法检测用绵羊红血球(SRBC)免疫过的小鼠脾细胞中抗体分泌细胞分泌抗SRBC抗体的功能,观察外科创伤对B细胞系统的抑制作用及抗体生成素对该抑制作用的纠正效应。材料和方法实验动物:健康雄性小鼠,体重18—22g,随机分为创伤组、创伤治疗组和正常对照组,每组10只。动物致敏:在实验前4天用10％SRBC 0.2ml,腹腔注射进行免疫。
In this experiment, plaque-forming cells (abbreviated as PEC) assay was used to detect the function of anti-SRBC antibodies secreted by antibody-secreting cells in mouse spleen cells immunized with erythrocyte sheep (SRBC) to observe the inhibitory effect of surgical trauma on B cell system and antibodies. The remedial effect of vasopressin on this inhibition. Materials and Methods Experimental Animals: Healthy male mice, weighing 18-22 g, were randomly divided into trauma group, trauma treatment group and normal control group, 10 in each group. Animal sensitization: Immunization was performed intraperitoneally with 10% SRBC 0.2 ml 4 days before the experiment.