为获得高分辨率、高灵敏度的2-DE图谱,以3叶1心期的水稻幼苗叶片为研究材料,对蛋白样本的制备、上样量及考马斯亮蓝R-250染色等关键步骤进行探讨,优化实验条件.结果表明,脱色后凝胶经5%冰乙酸浸泡后得到的2-DE图谱蛋白质点明显增多,图谱背景清晰.同时,不同公司考染试剂CBB R-250染色灵敏度存在较大的差别,应予以尝试.同其它方法相比,本方法简便、稳定,灵敏度高,可操作性强,对水稻蛋白质组学研究具有一定的指导意义. In order to obtain high-resolution and high-sensitivity 2-DE maps, key steps of preparation of protein samples, sample loading and Coomassie Brilliant Blue R-250 staining were explored with 3-leaf-1-stage rice seedling leaves as experimental material , The experimental conditions were optimized.The results showed that the protein spots of 2-DE map obtained after the gel was decolorized by 5% glacial acetic acid increased obviously, and the background of the spectrum was clear.At the same time, the sensitivity of CBB R- , Which should be tried.Compared with other methods, the method is simple, stable, sensitive and easy to operate, which is of guiding significance to the research of rice proteomics.