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以高浓度的赖氨酸、苏氨酸或色氨酸作为培养基添加物,对水稻体细胞和性细胞无性系进行离体筛选。获得一批耐高浓度氨基酸的抗性愈伤系并再生成绿色植株。据T_1代糙米氨基酸含量分析,9个突变体的17种氨基酸总量比原品种增加10.6～46.9％,7种必需氨基酸含量提高10.4～58.2％,其中赖氨酸提高7.8～42.3％。经主要农艺性状的变异系数分析,可育突变体在T_1～T_2代性状就相对稳定。本文讨论了抗性突变体离体筛选在水稻优质育种中的应用潜力。
High concentrations of lysine, threonine or tryptophan were used as media supplements to screen in vitro somatic and clonal cell clones of rice. A group of resistant calli with high concentrations of amino acids were obtained and green plants were regenerated. According to the amino acid content of brown rice in T_1 generation, the total amount of 17 amino acids in 9 mutants increased by 10.6-46.9% than the original varieties, the content of 7 essential amino acids increased by 10.4-58.2%, and the lysine increased by 7.8-42.3%. The analysis of variation coefficient of major agronomic traits indicated that the fertile mutant was relatively stable in T_1 -T_2 generation. This article discusses the potential of in vitro screening of resistant mutants in rice quality breeding.