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目的：在酶标板上进行ＤＮＡ探针杂交，评价其在检测临床标本中结核杆菌的应用价值。方法：收集７１份结核性标本和３２份非结核性标本，进行ＤＮＡ扩增，把扩增产物分别进行电泳和ＤＮＡ探针杂交，比较其结果。结果：７１份结核性标本电泳法阳性率为４５１％，ＤＮＡ探针法阳性率为６９０％；３２份非结核性标本二者均为阴性。结论：ＤＮＡ探针杂交可提高临床标本中结核杆菌的阳性检出率，是结核病早期诊断的一种新方法。
Objective: To carry out DNA probe hybridization on a microtiter plate to evaluate its application in the detection of Mycobacterium tuberculosis in clinical specimens. Methods: Seventy-one samples of tuberculous and 32 non-tuberculous specimens were collected for DNA amplification. The amplified products were respectively subjected to electrophoresis and DNA probe hybridization, and the results were compared. Results: The positive rate of 71 cases of tuberculous specimens was 451% and the positive rate of DNA probe method was 690%. Both of 32 non-tuberculous specimens were negative. Conclusion: DNA probe hybridization can improve the positive detection rate of M. tuberculosis in clinical specimens, which is a new method of early diagnosis of tuberculosis.