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目的:探讨白藜芦醇对异丙肾上腺素(ISO)诱导的乳鼠心肌细胞肥大的作用及其可能机制。方法:异丙肾上腺素10μmol/L诱导心肌细胞肥大,实验分为:正常组、异丙肾上腺素组、白藜芦醇25μmol/L组、白藜芦醇50μmol/L组、N-[2-[N-(4-氯肉桂)-N-甲基氨基]苯基]-N-(2-羟乙基)-4-甲氧苯磺酰胺磷酸酯盐(KN93)0.2μmol/L组。Lowry法测心肌细胞蛋白含量;鬼笔环肽染色测细胞大小;RT-PCR测细胞内ANP mRNA表达;Fura-2/AM为荧光探针,观察胞内[Ca2+]i瞬间变化,电泳法测定钙调蛋白激酶(Ca MKⅡ)蛋白表达。结果:异丙肾上腺素使心肌细胞蛋白含量增加,细胞体积增大,ANP mRNA表达增加,[Ca2+]i瞬变增加,Ca MKⅡ蛋白表达增高。与异丙肾上腺素组相比,白藜芦醇50μmol/L和KN93 0.2μmol/L组蛋白含量降低,细胞体积减少,ANP mRNA表达降低,[Ca2+]i瞬变降低,Ca MKⅡ蛋白表达降低。结论:白藜芦醇可通过降低胞内[Ca2+]i瞬变和Ca MKⅡ表达抑制异丙肾上腺素诱导的乳鼠心肌细胞肥大。
Objective: To investigate the effect of resveratrol on isoproterenol (ISO) -induced cardiomyocyte hypertrophy in neonatal rats and its possible mechanism. Methods: Myocardial cell hypertrophy was induced by 10μmol / L isoproterenol. The experiment was divided into normal group, isoprenaline group, resveratrol 25μmol / L group, resveratrol 50μmol / L group, N- [2- [N- (4-chlorocinnamyl) -N-methylamino] phenyl] -N- (2-hydroxyethyl) -4-methoxybenzenesulfonamide phosphate salt (KN93) 0.2 μmol / L group. Lowry method was used to measure the protein content of cardiomyocytes; phalloidin staining was used to measure cell size; RT-PCR was used to measure intracellular expression of ANP mRNA; Fura-2 / AM was used as fluorescent probe to observe intracellular [Ca2 +] i transient change; Calmodulin kinase (Ca MKII) protein expression. Isoprenaline increased the protein content of cardiomyocytes, increased the volume of cells, increased the expression of ANP mRNA, transiently increased [Ca2 +] i and increased the expression of CaMKII. Compared with isoproterenol group, the contents of resveratrol 50μmol / L and KN93 0.2μmol / L reduced the protein content, reduced the cell volume, decreased the expression of ANP mRNA, reduced the transient expression of [Ca2 +] i and decreased the expression of Ca2KII. CONCLUSION: Resveratrol inhibits isoprenaline-induced cardiomyocyte hypertrophy in neonatal rats by decreasing intracellular [Ca2 +] i transients and Ca MKII expression.