血小板(Plt)能够调节中性粒细胞(PMN)的反应和功能。作者分别用绵羊和人的Plt检测自体PMN产生超氧阴离子自由基(O_2~-)的调节作用。方法是在室温下,用塑料或硅化玻璃容器分离Plt,用低渗溶液除去红细胞后分离白细胞,得量分别为97％和95％,成活率≥98％。测定O_2~-时,将孵育后 Platelets (Plt) regulate the response and function of neutrophils (PMNs). The authors examined the regulation of superoxide anion free radicals (O 2 - -) by autologous PMN using sheep and human Plt. The method comprises the following steps: separating Plt from plastic or silicified glass container at room temperature; removing white blood cells after removing erythrocytes with hypotonic solution; the yield is 97% and 95%; the survival rate is more than or equal to 98%. O_2 ~ - determination, after incubation