论文部分内容阅读
目的通过检测HBsAg阳性产妇的血清及乳汁HBV-DNA载量,探讨产妇血清和乳汁HBV-DNA的相关性,以指导母乳喂养。方法选取2006年5月-2010年11月在产科住院分娩的HBsAg阳性产妇220例,采用荧光定量PCR检测产妇血清和乳汁HBV-DNA载量。结果①220例HBsAg阳性产妇中135例HBV-DNA阳性,阳性率为62.3%;220例中85例HBV-DNA阴性产妇的初乳、半月乳及满月乳HBV-DNA均阴性;135例HBV-DNA阳性产妇的初乳HBV-DNA检测26例阳性,阳性率为19.3%(26/135);余109例HBV-DNA阳性产妇复检半月乳HBV-DNA阳性47例,阳性率为43.1%(47/109);再余62例HBV-DNA阳性产妇复检满月乳HBV-DNA阳性35例,阳性率为56.5%(35/62);135例HBV-DNA阳性产妇中27例的初乳、半月乳及满月乳HBV-DNA均阴性,阴性率为20.0%(27/135)。②半月乳及满月乳分别与初乳比较,HBV-DNA阳性率均显著增高(χ2=16.375,27.317,P<0.01);半月乳(54.1%)及满月乳(80.0%)分别与初乳(19.3%)比较,HBV-DNA累计阳性率均显著增高(χ2=35.231,99.620,P<0.01)。结论血清HBV-DNA阴性产妇的乳汁是相对安全的,HBV-DNA阳性产妇乳汁中大多有HBV的存在,应避免母乳喂养,以防止传染。检测HBsAg阳性产妇乳汁HBV-DNA载量,可以直接反映产妇传染性的强弱,对指导母乳喂养提供了可靠依据。
Objective To detect the HBV-DNA load in serum and milk of HBsAg-positive pregnant women and explore the correlation between serum HBV-DNA in breast milk and breast milk to guide breastfeeding. Methods Totally 220 HBsAg-positive mothers who were delivered in obstetrics and gynecology hospital from May 2006 to November 2010 were selected. HBV-DNA levels in serum and milk of maternal were detected by fluorescence quantitative PCR. Results ① The positive rate of HBV-DNA in 135 cases was 62.3% in 220 cases of HBsAg-positive mothers. The HBV-DNA of 85 cases of HBV-DNA negative mothers in 220 cases were negative. HBV-DNA of 135 cases The positive maternal colostrum HBV-DNA test 26 cases were positive, the positive rate was 19.3% (26/135); more than 109 cases of HBV-DNA positive maternal retest semibutted HBV-DNA positive 47 cases, the positive rate was 43.1% (47 / 109). Of the remaining 62 HBV-DNA positive mothers, 35 were HBV-DNA positive in full moles, the positive rate was 56.5% (35/62) HBV-DNA in milk and full-term milk were all negative, with a negative rate of 20.0% (27/135). (2) The positive rates of HBV-DNA in half-moon milk and full-moon milk were significantly higher than those in colostrum (χ2 = 16.375,27.317, P <0.01) 19.3%), HBV-DNA cumulative positive rate was significantly higher (χ2 = 35.231,99.620, P <0.01). Conclusion Serum HBV-DNA negative maternal milk is relatively safe, HBV-DNA-positive maternal milk most of the presence of HBV, breastfeeding should be avoided to prevent infection. Detection of HBsAg-positive maternal milk HBV-DNA load, can directly reflect the strength of maternal infectious, to provide a reliable basis for the guidance of breastfeeding.