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目的探讨中药甘舒胶囊对抗氧化应激损伤的肝细胞保护作用。方法在Chang肝细胞建立氧化应激(H2O2)损伤的实验模型,应用甲氮甲唑蓝(MTT)检测法。PI染色流式细胞仪(FCM)及Hoechst 33258染色法等检测甘舒对抗H2O2诱导Chang肝细胞的细胞毒性及凋亡的细胞保护作用。结果H2O2呈浓度依赖性地降低Chang肝细胞的存活率;在自身不影响Chang肝细胞存活率的浓度(1～100μg/ml)范围内,甘舒呈浓度依赖性地对抗300μmol/L和400μmol/LH2O2对肝细胞存活率的抑制作用;另方面,在0～800μmol/L浓度范围内,H2O2呈浓度依赖性地增加Chang肝细胞的凋亡率;100μg/ml、500μg/ml和1mg/ml的甘舒本身不影响肝细胞的凋亡率,但却能显著地抑制300μmol/LH2O2诱导的肝细胞凋亡。结论中药甘舒胶囊具有抗氧化应激作用,可显著对抗氧化应激(H2O2)诱导的肝细胞损伤。
Objective To investigate the protective effect of Ganshu capsule against hepatic injury induced by oxidative stress. Methods The experimental model of oxidative stress (H2O2) injury was established in Chang liver cells, and MTT assay was used. PI staining flow cytometry (FCM) and Hoechst 33258 staining were used to detect the cytoprotective effect of Ganshu against H2O2-induced cytotoxicity and apoptosis in Chang liver cells. Results H2O2 decreased the survival rate of Chang hepatocytes in a concentration-dependent manner. Within the range of concentrations (1-100 μg/ml) that did not affect the survival rate of Chang hepatocytes, Ganshu responded to 300 μmol/L and 400 μmol/ml in a concentration-dependent manner. The inhibitory effect of LH2O2 on hepatocyte survival rate; On the other hand, H2O2 increased the apoptosis rate of Chang hepatocytes in a concentration-dependent manner from 0 to 800 μmol/L; 100 μg/ml, 500 μg/ml and 1 mg/ml. Ganshu itself does not affect the apoptosis rate of hepatocytes, but it can significantly inhibit 300μmol/LH2O2-induced hepatocyte apoptosis. Conclusion Ganshu capsule has anti-oxidative effect and can significantly protect against hepatic injury induced by oxidative stress (H2O2).