人组织型纤溶酶原激活剂(t-PA)是丝氨酸类蛋白水解酶,因它能特异地与纤维蛋白结合并受其激活,故被认为是一种高效的溶栓剂。本文结合作者的工作综述了近几年国内外在基因、蛋白质水平对t-PA进行研究的进展,深入讨论了t-PA分子结构与功能的关系,提出进行t-PA蛋白质工程以及利用抗体进行t-PA导向溶栓的可行性. Human tissue-type plasminogen activator (t-PA) is a serine proteolytic enzyme that is considered to be an efficient thrombolytic agent because it specifically binds to fibrin and is activated by it. In this paper, we review the progress of t-PA at home and abroad in recent years based on the work of the authors. The relationship between t-PA molecular structure and function was discussed in depth. The t-PA protein engineering and the use of antibodies were proposed Feasibility of t-PA-guided thrombolysis.