本试验用三种类型的小麦幼穗为材料,在幼穗的发育时期,去分化和分化培养基等完全相同的条件下,对它们在离体培养下的反应进行了比较,发现愈伤组织的诱导率和苗的分化率以及愈伤组织在继代培养中的行为,都因不同类型的小麦幼穗而产生显著差异,而且愈伤组织的诱导率和苗的分化率之间不存在相关性。我们认为,材料的基因型选择,可能是建立小麦的胚性细胞悬浮培养物系统的一个有希望的途径。 In this experiment, three types of young wheat ears were used as materials to compare their response to in vitro culture under the same conditions of young panicle development, de-differentiation and differentiation medium. The results showed that callus The induction rate and differentiation rate of seedlings and the callus in subculture were all significantly different due to different types of young ears and there was no correlation between callus induction rate and seedling differentiation rate Sex. We believe that the choice of genotypes for the material may be a promising way to establish an embryogenic cell suspension culture system for wheat.