论文部分内容阅读
用苹果试管苗Ｍ２６作砧木，‘Ｇａｌａ’作接穗，成功地获得试管嫁接苗。嫁接苗在ＭＳ液体纸桥培养基上的生长量与嫁接成活率显著高于ＭＳ固体培养基。但是，液体培养基导致砧木基部膨大，形成玻璃苗。随ＢＡＰ浓度升高，嫁接部位愈伤组织形成快、成活率高，接穗生长量明显增加。过高浓度的ＢＡＰ（≥０．８ｍｇ·１~(-１)），诱导砧木侧枝大量形成、砧木基部膨大、产生玻璃苗，并且显著降低砧木的生根力。试管嫁接苗建立阶段最适的ＢＡＰ浓度为０．４ｍｇ·１~(-１)。试管嫁接苗的生根力随ＩＢＡ浓度升高而显著提高，在１．０ｍｇ·１~(-１)浓度时达最适水平。ＩＢＡ浓度对试管嫁接苗接穗生长的影响与生根力是一致的。
The test-tube grafted seedlings were successfully obtained using apple test tube M26 as rootstock and ’Gala’ as the scion. Grafted seedlings on MS liquid paper bridge growth and grafting survival rate was significantly higher than MS solid medium. However, the liquid medium causes the base of the rootstock to expand to form glass seedlings. With the increase of BAP concentration, the callus of the grafted area formed fast, the survival rate was high, and the amount of scion growth was significantly increased. High concentrations of BAP (≥0.8 mg · 1 -1) induced large quantities of collateral of rootstocks, enlargement of base of rootstocks, production of glass seedlings, and significantly reduced the rooting ability of rootstocks. The optimum concentration of BAP in the stage of in vitro grafting was 0.4mg · 1 ~ (-1). The rooting ability of in vitro grafted seedlings increased significantly with the increase of IBA concentration, reaching the optimum level at 1.0 mg · 1 -1 concentration. The effect of IBA concentration on the growth of scion in vitro was consistent with that of rooting.