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利用有限稀释法,进行人低分化鼻咽癌上皮细胞系(CNE—2Z)的单克隆化培养,成功地建立了25个单克隆细胞株,并根据细胞电泳率、细胞体外培养的倍增时间、分裂指数及低血清培养等筛选指标,从中筛选出电泳率差异较大及体外生长特性有明显差异的两个克隆化细胞株(F1、H5)。将该两株及母系的癌细胞分别接
Monoclonal culture of human poorly-differentiated nasopharyngeal epithelial cell line (CNE-2Z) was performed using the limiting dilution method. Twenty-five monoclonal cell lines were successfully established and based on the cell electrophoretic rate, doubling time of cell culture in vitro, Fractionation index and low serum culture and other screening indicators were used to screen two cloned cell lines (F1, H5) with large difference in electrophoresis rate and in vitro growth characteristics. Connect the two strains with the maternal cancer cells